Biology and medicine are undergoing a tremendous revolution in which the use of advanced technologies, sophisticated bioinformatics and big data can immediately impact disease diagnosis, treatment and outcome. Thus, the main objective of this project, which combines clinical and basic scientific skills, is to extend our knowledge of the pathways controlling human immunity in early life, to increase the number of primary immunodeficiency diseases to be diagnosed early in life, to understand new disease patho-mechanisms, and to develop a novel and personalized gene-based treatment.